Improvement of arbovirus HA antigens by treatment with a colloidal silica gel and sonication
Identifieur interne : 001767 ( Main/Exploration ); précédent : 001766; suivant : 001768Improvement of arbovirus HA antigens by treatment with a colloidal silica gel and sonication
Auteurs : T. Traavik [Norvège]Source :
- Archives of Virology [ 0304-8608 ] ; 1977-09-01.
English descriptors
- Teeft :
- Acta path, Aerosil, Aerosil absorption, Aerosil treatment, Antigen, Arbovirus, Beneficial effect, Borate saline, Brain suspensions, Cell culture, Cell culture fluids, Cell cultures, Colloidal silica, Culture fluids, Highest titers, Polyethylene glycol, Reciprocal values, Silica, Simple method, Sonication, Suckling, Suckling mice, Suckling mouse brains, Titer.
Abstract
Summary: A remarkable increase in HA titers for weakly haemagglutinating Norwegian arbovirus strains, Uukuniemi and Runde viruses, was achieved by including treatment with the colloidal silica gel Aerosil in the antigen preparation scheme. By combining this procedure with sonication, the titers of sucrose-aceton extracted, infected suckling mouse brains could be increased several hundred times. Good antigens also were obtained from virus grown in BHK 21/c 13 cell cultures and concentrated by polyethylene glycol 6000/NaCl. Rubella virus HA antigen and HBsAg were adsorbed to the gel, and excluded from a preparation by treatment with Aerosil. This indicates a limitation to the universal use of the method, presumably related to the particle size.
Url:
DOI: 10.1007/BF01314788
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000490
- to stream Istex, to step Curation: 000470
- to stream Istex, to step Checkpoint: 000869
- to stream Main, to step Merge: 001799
- to stream Main, to step Curation: 001767
Le document en format XML
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